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Extra info for Aplastic Anemia - A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References
Aspects of endocrine and somatic abnormality are being related to accumulated data regarding the mutations responsible for FA. Two papers relating to this protocol have been accepted for publication in peerreviewed journals. ; Pathology and Lab Medicine; University of Wisconsin Madison 750 University Ave Madison, Wi 53706 Timing: Fiscal Year 2003; Project Start 01-MAR-2003; Project End 31-JAN-2008 Summary: (provided by applicant): The molecular aspects of the earliest steps in embryonic stem (ES) cell differentiation remain poorly understood.
In addition, we will use microcellmediated chromosome transfer to map additional FA genes and positionally clone them in the future. We have generated knock-out mice for FA group C and will use these animals to study the role of FANCC in DNA cross-link responses in vivo, the biology of hematopoietic stem cells in FA and as a model FANCC in DNA cross-link responses in vivo, the biology of hematopoietic stem cells in FA and as a model for preclinical gene therapy. We will also generate knock-out mice for FA groups A and D.
Based on this hypothesis, the specific aims of this project will continue to: investigate biochemical and molecular interactions of BPderived quinones and BP-7,8-dihydrodiol (BP-diol) which occur as a result of their interactions with organelles and enzymes from bone marrow cells; investigate the mechanisms of toxicity of BP and its metabolites to DBA/2 bone marrow stromal cells in vitro and in vivo; examine the ability of BP and its metabolites to alter the differentiation of human myeloid cell lines, ML-1 and HL-60, and progenitor cells from DBA/2 mice; and evaluate if a peroxide-dependent mechanism is involved in the bioactivation of BPdiol in vitro and in vivo.