By K. Jinno
This can be the 1st e-book to target the newest advancements in hyphenated suggestions utilizing supercritical fluids. the benefits of SFC in hyphenation with numerous detection modes, corresponding to FTIR, MS, MPD and ICP and others are in actual fact featured in the course of the booklet. targeted awareness is paid to coupling of SFE with GC or SFC. during this edited quantity, chapters are written through prime specialists within the box. The ebook could be of curiosity to pros in academia, in addition to to these researchers operating in an business surroundings, similar to analytical instrumentation, prescription drugs, agriculture, meals, petrochemicals and environmental.
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Extra info for Hyphenated Techniques in Supercritical Fluid Chromatography and Extraction
53 HYPHENATION BETWEEN ELSD AND SEMI MICRO PACKED COLUMN SFC The light scattering detector has been used so far mainly for liquid chromatography to analyze triglycerides, phospholipids, sugars, steroids, fatty acids and oligomers of surfactants as ethoxylate alcohol. [ 9 -131 Recent attempts to use ELSD on SFC by means of direct coupling ELSD to the SFC column have been reported. d. stainless tubing. The ELSD, Varex Mark IIA, was used without further modification. For making proper aerosol, it was necessary to increase the flow rate of a supplemental rinse solvent which had been set around 100ul/min in normal run with a UV detector, to about 500 fil/min.
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This information obtained by UV-ELSD might be valuable for discussing whether the structure of additive unit of oligomer has UV-active function group or not. 1 EL^^ __1 I 1 u UV D I 0 I0 n 20 min Fig. 5 Temperature gradient elution of nonionic surfactant, Triton X-100, by UV-ELSD hyphenation system. d. x 500 mm length packed with ODs-5. The other experimental conditions are shown in the text.