By José M. Guisán
The second one version of Immobilization of Enzymes and Cells serves as an replace in addition to a praise to the 1st variation. This quantity now comprises uncomplicated protocols for the immobilization of enzymes and cells that may be invaluable for program at business scale, novel protocols for immobilization sooner or later, and new chemical reactors capable of triumph over the restrictions of a few immobilized derivatives.
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Additional resources for Immobilization Of Enzymes And Cells (Methods in Biotechnology)
Example text
Indeed, penicillin G acylase CLEAs, prepared by precipitation with, for example, ammonium sulfate or tertbutanol, proved to be effective catalysts for the synthesis of ampicillin (15). They exhibited activities comparable with those of CLECs of the same enzyme, with substantially less competing hydrolysis of the side chain. Analogous to the corresponding CLECs, the penicillin G acylase CLEAs also maintained their activity in organic solvents. We then examined the effect of various parameters, such as the precipitant and the addition of additives such as surfactants and crown ethers, on the activities of CLEAs prepared from seven commercially available lipases (16).
The method is simple and reversible but, in general, it is difficult to find conditions under which the enzyme remains both strongly bound and fully active. More recently, the use of immobilized polymeric-ionic ligands has allowed for modulation of protein– matrix interactions and has thus optimized the properties of the derivative. A number of patents have been filed on the use of polyethyleneimine to bind a rich variety of enzymes and whole cells (43). However, problems may arise from the use of a highly charged support when the substrates or products themselves are charged; the kinetics are distorted as a result of partition or diffusion phenomena.
20. The filter system was supplied by CUNO Benelux. 5 µm Zeta Plus 050 HT filter were used. CLEAS 41 3. 1. Cross-Linked Enzyme Aggregate Activity (see Note 2) 1. For the cross-link optimization 90 µL precipitant containing glutaraldehyde in varying concentrations was added to 10 µL enzyme solution (see Note 3). 2. The samples were incubated at room temperature without shaking. For the volumetric activity optimization 90 µL precipitant containing glutaraldehyde was added to 10 µL solution with a varying protein content and after the specific cross-linking time this mixture was quenched with 900 µL buffer.
