By Gerald H. Wagman and Marvin J. Weinstein (Eds.)
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Extra resources for Chromatography of Antibiotics
Toda, Y. Abe, M. Oka, H. Yamashita, T. Yamasaki, K. Fujisawa and H. Kawaguchi, J. 27 Ref. S. Nakamura, Phenol-water (80: 20) H. Umezawa and N. Ishida, J. , 15 (1961) 163-164 ReJ J. Berger, D. P. Scannell. Patent 3,775,255, Nov. Patent 3,751,459, AMIDOMYCIN Aug. 7, 1973 PC TLC 2 Solvents A. Light petroleum vs. paper impregnated with Medium ethylene glycol B. n-Amy1 alcohol satd. with water C. Ethanol-acetic acid-water (3 : 1 :6) D. Ethanol-water (2: 3) Detection Bioautography vs. Candida albicans Solvent Silica gel glass plates Ethanol-water-ammonia (49: 49: 2) Detection Ninhydrin Ref.
Finally, chromatography of antibiotic hydrolysates followed by a ninhydrin spray is a useful “fingerprint” technique for comparison of similar substances, most particularly for identification of aminoglycosides. 0). This can be mounted with the zero end held fast to a strip of metal and a movable clamp attached to the opposite end of the tape. 0” placed at the front. The R, can then be read directly from the scale opposite the antibiotic zone. A rapid, simple means of recording chromatographic data is the use of Polaroid equipment.
Paper impregnated with Medium ethylene glycol B. n-Amy1 alcohol satd. with water C. Ethanol-acetic acid-water (3 : 1 :6) D. Ethanol-water (2: 3) Detection Bioautography vs. Candida albicans Solvent Silica gel glass plates Ethanol-water-ammonia (49: 49: 2) Detection Ninhydrin Ref. , cephalosporins) TLC Medium Silica gel Solvents A . 5:21) B. n-Butanol-pyridine-glacial acetic acid-water (42: 24: 4: 30) Ref. H. Bickel, J. Bosshardt, H. Peter and B. S. Patent 3,646,023, Feb. 44 zone corresponds to 3-amino-3-deoxy-~-ghcose.