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Methods in High Resolution Separation and Analysis of by John N. Abelson, Melvin I. Simon, Barry L. Karger, William

By John N. Abelson, Melvin I. Simon, Barry L. Karger, William Hancock

The significantly acclaimed laboratory typical for greater than 40 years, equipment in Enzymology is among the such a lot hugely revered courses within the box of biochemistry. when you consider that 1955, every one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. greater than 260 volumes were released (all of them nonetheless in print) and lots more and plenty of the fabric is appropriate even today--truly a necessary ebook for researchers in all fields of lifestyles sciences. Key gains* Liquid chromatography* Electrophoresis* Mass spectrometry

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S L. R. Snyder, J. W. Dolan, and D. C. Lommen, in "HPLC of Peptides and Proteins: Separation, Analysisand Conformation" (C. T. Mant and R. S. ), p. 725. CRC Press, Boca Ratom FL, 1991. 12~L. R. Snyder, Methods Enzymol. 270, Chap. 7. 1996. i~ R. W. Giese, J. K. Haken, K. Macek, and L. R. "J. Chromatogr. 485,ElsevieL Amsterdam,The Netherlands, 1989. [2] PEPTIDE MAPPINGBY RP-HPLC 51 [21 A n a l y t i c a l P e p t i d e M a p p i n g o f R e c o m b i n a n t DNA-Derived Proteins by Reversed-Phase High-Performance Liquid Chromatography By EDWARD R.

W. L. Burke, C. T. Mant, and R. S. Hodges, in "HPLC of Peptides and Proteins: Separation, Analysis and Conformation" (C. T. Mant and R. S. ), p. 307. CRC Press, Boca Raton, FL, 1991. 2" o $4 $2~ uJ 0 z < m nO t0 O- F T T 0 10 20 30 ELUTION TIME (min) Ft(;. 7. M(miĀ¢ori~ago(reversed-phase column performance with synthetic peptide standards under recommended standard conditions. 5->m particle size, 300-,~ pore size; SynChrom, Lafayette, IN), Instrumentation: Same as in Fig. 1, except for a Hewlett-Paekard HP 10t)(}liquid chromatograph (including autosampler) and an HP9000 Series 300 computer.

Lozzi, M. Rustici, A. Santucci, L. Bracci, S. Petreni, P. Soldani, and P. Neri. J. Liq. Chromatogr. 11, 1651 (1988). 77 G. R. Rhodes, M. J. Rubenfield. and C. T. Garvie, J. Chromatogr. 488, 456 (1989). 7s L(F-GC 10, 515 (1992). > D. Guo. C. T. Mant, and R. S. I. (Tzromatogr. 386, 2115 (1987). so C. T. Manl and R. S. Hodges. in "'HPLC of Peptides and Proteins: Separation, Analysis and C o n f o r m a t i o n " (('. T. Mant and R. S. ), p. 327. C R C Press, Boca Raton, F L 1991. s~ H. P. J. B e n n e n , J.

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