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Cardiovascular Research: New Technologies, Methods, and by Branko Braam, Hans Bluyssen (auth.), Gerard Pasterkamp,

By Branko Braam, Hans Bluyssen (auth.), Gerard Pasterkamp, Dominique P. V. de Kleijn (eds.)

Cardiovascular study: New applied sciences, tools & Applications serves as a vital reference fabric supplying an entire evaluate of cutting edge new applied sciences, equipment, and purposes in present cardiovascular learn. Cardiovascular learn: New applied sciences, equipment & Applications provides a equipment and functions method of cardiovascular learn written via admired foreign researchers featuring commissioned summaries of innovative research.

Key Topics:

Offers a tools and functions process together with, Cardiovascular tools in:



-Real Time PCR

-RNA Interference

-Plus More!

Cardiovascular functions in:

-Stems Cells and Cardiomyocytes

-Stem Cells and Angiogenesis

-Gene Therapy

-Manufacturing the substitute Blood Vessel

-Closed Chest robot Surgery

-RNA Interference

-Plus More!

Cardiovascular examine: New applied sciences, tools & Applications is vital for all cardiovascular study lecturers, bench scientists, and pros wishing to use the newest and maximum within the always rising box of cardiovascular technological know-how.

About the Editors:

Gerard Pasterkamp, PhD, MD is affiliate Professor and Head of Experimental Cardiology Laboratory on the middle Lung Centre Utrecht on the college clinical middle Utrecht (UMCU). Dr. Pasterkamp got his MD at loose college Amsterdam, his PhD on the scientific college on intravascular ultrasound, and his MSc medical Epidemiology on the NIHES Rotterdam. Dr. Pasterkamp's many unique papers and invited experiences relating to vascular home improvement show his expert curiosity in arterial home improvement in atherosclerotic sickness.

Dominique de Kleijn, PhD is a molecular biologist at Interuniversity Cardiology Institute of the Netherlands (ICIN) and is located on the Laboratory of Experimental Cardiology on the UMCU. Dr. Klein earned his PhD molecular biology on the Nijmegen college on the division of Molecular Animal body structure, and is famous as a professional in matrix biology and innate immunity in heart problems.

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2000). , 2002). Remarkably, there have been extremely few attempts to classify cardiovascular disease from gene expression profiles. 2. 1. Cross-sectional Experiments versus Time Course Experiments Cross-sectional experiments are relatively simple and extremely suitable for comparisons where one (or more) factor(s) is unambiguously present or absent. Such comparisons have been applied in oncology to test cancerous versus healthy tissue or to compare tissues from genetically different animals. , 2000).

2000). , 1999). Historically, the robotically spotted microarrays were referred to as cDNA microarrays because the nucleic acids being spotted were PCR products amplified from cDNA libraries. , 2003). A typical spotted array is printed onto a 60 mm by 20 mm glass microscope slide by a computer-controlled spotting robot; each spot is about 50–150 µm in diameter. Nucleotide arrays are usually spotted on glass slides but can also be produced on a nylon membrane with spots of slightly larger size in a lower density pattern.

Diagram for validation approach. of known genes and/or interrogate many ESTs. g. cytokines, in a known pathway. For such purposes there are affordable commercial arrays available (and one could even imagine testing a set of genes with Northerns or real-time PCR). When the response to a stimulus is studied that is known to exert very broad expressional actions, but the aim is still to study the behavior of known genes, one can use a large microarray. If the specific aim is to identify new genes, the probe set should contain a large number of ESTs.

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