By Lucienne Chatenoud (auth.), Maria Cristina Cuturi, Ignacio Anegon (eds.)
Over the earlier numerous years, a excessive variety of regulatory cells and suppressive molecules has taken centre degree within the box of immunoregulation. In Suppression and rules of Immune Responses: tools and Protocols, professional researchers spotlight contemporary advances within the id, characterization, and iteration of regulatory cells not just of the T cellphone lineage but additionally of alternative origins comparable to B, NK, myeloid, and dendritic cells, in addition to the position of numerous suppressive molecules in immunoregulation. specific emphasis is put on the characterization of the molecular mechanisms and the healing purposes of regulatory cells and molecules in human ailments. Written as a quantity within the hugely winning tools in Molecular Biology™ sequence, this paintings offers the type of special description and implementation recommendation that's an important for buying optimum effects. complete and state of the art, Suppression and legislation of Immune Responses: tools and Protocols serves as a key reference for scientists looking a manner towards better keep an eye on over the big energy of the complicated and important immune system.
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Additional resources for Suppression and Regulation of Immune Responses: Methods and Protocols
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15. 16. 17. Hoffmann, Eder, and Edinger CD4(+)CD25(+) regulatory T cells suppress lethal acute graft-versus-host disease after allogeneic bone marrow transplantation. J Exp Med 196:389–99. Hoffmann P, Boeld TJ, Eder R, Albrecht J, Doser K, Piseshka B, Dada A, Niemand C, Assenmacher M, Orso E, Andreesen R, Holler E, and Edinger M (2006) Isolation of CD4(+) CD25(+) regulatory T cells for clinical trials. Biol Blood Marrow Transplant 12:267–74. Trzonkowski P, Bieniaszewska M, Juscinska J, Dobyszuk A, Krzystyniak A, Marek N, Mysliwska J, and Hellmann A (2009) First-inman clinical results of the treatment of patients with graft versus host disease with human ex vivo expanded CD4+CD25+CD127- T regulatory cells.
Data represent mean ± SD from four individual cultures. 4. 6. 1. Staining of In Vitro Expanded Treg Cells for FOXP3 with or without Simultaneous Staining for Proinflammatory Cytokines FOXP3 expression is still the most widely used and generally accepted quality control criterion for in vitro-expanded Treg cells. However, since FOXP3 can also be transiently expressed by activated Tconv cells, a short resting period before staining for FOXP3 is recommended. Alternatively, expanded cells can be simultaneously stained for FOXP3 and proinflammatory cytokines to reveal any cells converting from a Treg to a Tconv phenotype during in vitro culture.
References 1. , and K. Kondo. 1971. Infec tious immunological tolerance. Immunology 21:903–914. 2. , N. Sakaguchi, M. Asano, M. Itoh, and M. Toda. 1995. Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25). Breakdown of a single mechanism of selftolerance causes various autoimmune diseases. J Immunol 155:1151–1164. 3. , and A. Rudensky. 2006. The role of the transcription factor Foxp3 in the deve lopment of regulatory T cells. Immunol Rev 212:86–98.
