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Prostate Cancer Methods and Protocols (Methods in Molecular by PJ Russell, P Jackson, EA Kingsley

By PJ Russell, P Jackson, EA Kingsley

A suite of simple and complicated molecular tools that display these markers crucial for extra actual diagnoses of particular illnesses, and in constructing new therapy ideas. The options diversity from in vitro the way to in vivo types of prostate melanoma, and contain new equipment for the exact analysis of prostate melanoma, proteome and microarray analyses, and new techniques for the therapy of refractory disorder. each one strategy is defined in step by step element to make sure winning effects and stay away from failure.

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2000) Stepwise genetic changes associated with progression of nontumorigenic HPV-18 immortalized human prostate cancer-derived cell line to a malignant phenotype. Cancer Genet. Cytogenet. 120, 117–126. 65. Webber, M. , Thraves, P. , Chu, W. , Storto, P. , et al. (1999) A human prostatic stromal myofibroblast cell line WPMY-1: A model for stromal-epithelial interactions in prostatic neoplasia. Carcinogenesis 20, 1185–1192. 66. Bright, R. , Vocke, C. , Emmert-Buck, M. , Duray, P. , et al. (1997) Generation and genetic characterization of immortal human prostate epithelial cell lines derived from primary cancer specimens.

Store at 4°C for up to 2 mo. 7. , Campbell, CA) and swirl to dissolve. Then, transfer 1 mL of this 1 mg/mL solution to a sterile tube containing 9 mL of sterile H2O; mix. Transfer 1-mL aliquots of this 100 µg/mL solution to sterile tubes and store at 4°C. CT should not be frozen. 8. Epidermal Growth Factor (EGF) The procedure for EGF is as follows: add 1 mL of sterile H2O to a sterile vial containing 100 µg of EGF (Collaborative Biomedical Products, Bedford, MA). Swirl to dissolve, and transfer 100-µL aliquots of this 100 µg/mL solution to sterile tubes.

40, 833–839. 37. Liu, X. , Wiley, H. , and Meikle, A. W. (1993) Androgens regulate proliferation of human prostate cancer cells in culture by increasing transforming growth factor-α (TGF-α) and epidermal growth factor (EGF)/TGF-α receptor. J. Clin. Endocrinol. Metab. 77, 1472–1478. 38. Loop, S. , Rozanski, T. , and Ostenson, R. C. (1993) Human primary prostate tumor cell line, ALVA-31: A new model for studying the hormonal regulation of prostate tumor cell growth. Prostate 22, 93–108. 39. Zhuang, S.

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