By John Swansbury
A suite of key cytogenetic and FISH suggestions utilized by glossy medical laboratories within the genetic research of human malignancies. The book's useful suggestion and strategies are appropriate to be used at each point of workmanship, together with absolutely demonstrated laboratories, yet with a sympathetic bias in the direction of a person contemplating constructing a brand new cytogenetics carrier. right here the reader will locate not just straight forward tutorials at the basics of human karyotypes and chromosome research, but in addition specified discussions on how laboratories could optimally improve their repertoire of features to incorporate such more moderen complementary thoughts as CGH, FISH, and M-FISH.
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Additional resources for Cancer Cytogenetics. Methods and Protocols
Example text
1995) Persistence of RAR alpha-PML fusion mRNA detected by reverse transcriptase polymerase chain reaction in patients in long-term remission of acute promyelocytic leukemia. Br. J. Haematol. 90(3), 615–618. 28. Licht, J. , et al. (1995) Clinical and molecular characterization of a rare syndrome of acute promyelocytic leukemia associated with translocation (11;17). Blood 85, 1083–1094. 29. Wong, K. F. and Kwong, Y. L. (1999) Trisomy 22 in acute myeloid leukemia: a marker for myeloid leukemia with monocytic features and cytogenetically cryptic inversion 16.
If the sample has clotted, see Note 1. 2. Choice of Cultures It has been shown that in samples of myeloid disorders, erythropoietic divisions predominate in the first few hours of culture, with granulopoietic divisions appearing subsequently (see Chapter 2). This corresponds to the observation that for the rare erythroleukemia (AML-M6), short-term cultures (harvested the same day that the sample was taken) are more likely to have clonal divisions. , overnight; see Note 2). As described in Chapter 2, several cultures should be set up if there is sufficient material is available, because the cell cycle time is unpredictably affected by the disease.
And Yin, J. A. (1995) Persistence of RAR alpha-PML fusion mRNA detected by reverse transcriptase polymerase chain reaction in patients in long-term remission of acute promyelocytic leukemia. Br. J. Haematol. 90(3), 615–618. 28. Licht, J. , et al. (1995) Clinical and molecular characterization of a rare syndrome of acute promyelocytic leukemia associated with translocation (11;17). Blood 85, 1083–1094. 29. Wong, K. F. and Kwong, Y. L. (1999) Trisomy 22 in acute myeloid leukemia: a marker for myeloid leukemia with monocytic features and cytogenetically cryptic inversion 16.
