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S L. R. Snyder, J. W. Dolan, and D. C. Lommen, in "HPLC of Peptides and Proteins: Separation, Analysisand Conformation" (C. T. Mant and R. S. ), p. 725. CRC Press, Boca Ratom FL, 1991. 12~L. R. Snyder, Methods Enzymol. 270, Chap. 7. 1996. i~ R. W. Giese, J. K. Haken, K. Macek, and L. R. "J. Chromatogr. 485,ElsevieL Amsterdam,The Netherlands, 1989. [2] PEPTIDE MAPPINGBY RP-HPLC 51 [21 A n a l y t i c a l P e p t i d e M a p p i n g o f R e c o m b i n a n t DNA-Derived Proteins by Reversed-Phase High-Performance Liquid Chromatography By EDWARD R.
W. L. Burke, C. T. Mant, and R. S. Hodges, in "HPLC of Peptides and Proteins: Separation, Analysis and Conformation" (C. T. Mant and R. S. ), p. 307. CRC Press, Boca Raton, FL, 1991. 2" o $4 $2~ uJ 0 z < m nO t0 O- F T T 0 10 20 30 ELUTION TIME (min) Ft(;. 7. M(mi¢ori~ago(reversed-phase column performance with synthetic peptide standards under recommended standard conditions. 5->m particle size, 300-,~ pore size; SynChrom, Lafayette, IN), Instrumentation: Same as in Fig. 1, except for a Hewlett-Paekard HP 10t)(}liquid chromatograph (including autosampler) and an HP9000 Series 300 computer.
Lozzi, M. Rustici, A. Santucci, L. Bracci, S. Petreni, P. Soldani, and P. Neri. J. Liq. Chromatogr. 11, 1651 (1988). 77 G. R. Rhodes, M. J. Rubenfield. and C. T. Garvie, J. Chromatogr. 488, 456 (1989). 7s L(F-GC 10, 515 (1992). > D. Guo. C. T. Mant, and R. S. I. (Tzromatogr. 386, 2115 (1987). so C. T. Manl and R. S. Hodges. in "'HPLC of Peptides and Proteins: Separation, Analysis and C o n f o r m a t i o n " (('. T. Mant and R. S. ), p. 327. C R C Press, Boca Raton, F L 1991. s~ H. P. J. B e n n e n , J.
