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Modern Size-Exclusion Liquid Chromatography: Practice of Gel by Andre Striegel, Wallace W. Yau, Joseph J. Kirkland, Donald

By Andre Striegel, Wallace W. Yau, Joseph J. Kirkland, Donald D. Bly

The Second Edition of Modern Size-Exclusion Chromatography deals an entire advisor to the theories, equipment, and purposes of size-exclusion chromatography. It offers an unheard of, built-in, up to date remedy of gel permeation and gel filtration chromatography. With its special descriptions of innovations, info dealing with, compilations of data on columns and column packings, and tables of significant solvents and reference fabrics, the publication deals readers every thing they should take complete benefit of this well known macromolecular characterization procedure.

Since ebook of the 1st version in 1979, there were many very important advances within the box of size-exclusion chromatography. This moment version brings the e-book completely brand new, with specialist assurance of:

  • New and rising business and examine applications
  • Practical elements of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC applied sciences for polymer structure and copolymer analysis
  • Updated info at the most recent gear and techniques
  • New top practices for the lab
  • SEC in terms of polymer characterization thoughts corresponding to GPEC, LCCC, and rheology

Throughout the textual content, distinct examples advisor you step-by-step via the entire most modern ideas and functions. With its wide revisions and updates written through major specialists and pioneers within the box, sleek Size-Exclusion Liquid Chromatography is still the definitive source for the wide variety of researchers and scientists who use HPLC and GPC methods.

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Extra info for Modern Size-Exclusion Liquid Chromatography: Practice of Gel Permeation and Gel Filtration Chromatography, 2nd Edition

Sample text

8 are preferable to values of VR for describing SEC peak retention. The values of K SEC compensate for column-size variations. For any SEC column, regardless of size, K SEC = 0 at exclusion and K SEC = 1 at total permeation. 3 illustrates the gradual approach of the usual experimental SEC calibration curve to the column exclusion and permeation limits. The solid straight line, the linear approximation to the calibration curve, is commonly used in SEC to facilitate MMD calculations (Chapter 8). It should be noted that peak A may be comprised of a number of solutes, all of size greater than the largest pore volume.

Humphrey, Polym. Eng. , 16, 11 (1976). R. Koningsveld, Adv. Polym. , 7, 1 (1970). A. M. Striegel, Anal. Bioanal. , 390, 303 (2008). 1 INTRODUCTION In column chromatography, sample components migrate through the column at different velocities and elute separately from the column at different times. As a solute moves along with the carrier fluid (mobile phase), it is at times held back momentarily either by the surface of the column packing, by a contained stagnant phase of the column packing (stationary phase), or by both.

Ser. 893, American Chemical Society, Washington, DC, 2005. A. M. Striegel, Anal. , 77, 104A (2005). A. M. Striegel, in Ref. 18, Chap. 1. A. M. Striegel and J. D. Timpa, in Strategies in Size Exclusion Chromatography, ACS Symp. Ser. 635, M. Potschka and P. L. , American Chemical Society, Washington, DC, 1996, Chapter 20. B. J. Catley and W. J. Whelan, Arch. Biochem. , 143, 138 (1971). F. W. , Wiley, New York, 1984. A. M. Striegel, Anal. , 74, 3013 (2002). A. M. Striegel, Polym. , 52, 1863 (2003).

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