By David Glick
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Additional info for Methods of Biochemical Analysis, Volume 8
Sample text
Results of oxidation of monounsaturated Cls acids from cow’s milk. Conditions as for Figure 22. Peak identification: (1)air, (2) methyl n-pentsnoate, (3) methyl n-hexanoate, (4) dimethyl succinate, (5) methyl n-heptanoate, (6) dimethyl glutarate, (7) methyl n-octanoate, (8) dimethyl adipate, (9) methyl n-nonanoate, (10) dimethyl pimelate, (11) dimethyl suberate, (12) dimethyl azela-ate, (13) dimethyl sebacate, (14) dimethyl hendecanedioate, (15) methyl n-tetradecanoate, ( 16) methyl n-pentadecanoate, (17) dimethyl tridecanedioate.
36 Apieson L at 197OC. 93 - PolyPolyethylene diethylene glycol glycol Apieson M Reoplex 400 adipate succinate a t 197°C. a t 197°C. a t 180°C. at 203°C. 2- No. of carbon atoms Fig. 18. Relationship between loglo retention volume and number of carbon atoms for the free acids from C1 to C12. The first two points do not fall on the straight line. 1 . 0 1 - 2 o L 6 Number of carbon otorns Fig. 19. og,o relative retention volume and chain length for the long chain fatty acid esters from Ca to CPPon two types of column: Apieron L a t 200°C.
A micropipet holding approximately 5 pl. is filled with solvent in the boiling tube and placed on the lens tissue between the perforations of the planchette. A t each filling the micropipet is placed on the next gap and so on in a circular manner so that the sample is distributed evenly over the disc. Evaporation of solvent takes place over the perforations rather than the edges of the disc, so that the fatty acid sample is concentrated in spots over the holes. With care, an even distribution results.
