By Michael A. Madson
Mass Spectrometry: innovations for the Structural Characterization of Glycans provides new tools for accomplishing designated carbohydrate qualitative analysis―arming analytical chemists, pharmaceutical scientists, and nutrients scientists with a short reference that would let them make sure the constructions of carbohydrates molecules.
As there's a want within the medical neighborhood for content material particular to structural selection and research of recent glycoprotein drug, and since structure-activity research calls for a structural decision of the N- and O-linked oligosaccharides associated with glycol-proteins, this e-book presents the correct learn which are beneficial for advances and new results during this quarter of analysis.
- Authored via an analytical chemist with greater than 30 years of expertise in learn and industry
- Serves as a brief reference in mass spectral research and carbohydrates
- Includes greater than 60 figures to help within the retention of key concepts
Read or Download Mass Spectrometry. Techniques for Structural Characterization of Glycans PDF
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Extra resources for Mass Spectrometry. Techniques for Structural Characterization of Glycans
Example text
98). 99 We have here a carboxylium ion of sialic acid, substituted on the hexosyl structure at the 6' position. There is dehydrated, hydride inserted sulfate at the 40 position and an unknown substitution at 30 of the hexosyl group, possible sialic acid as substitution, and substitution at the 6 position, possibly a dihydrido sulfo substitution (Fig. 99). This structure proposes both the hydride inserted and the reduced hexosaminitol and is evidence that the smaller of the two peaks is disulfated (Fig.
87). Here we find the whole molecule fragmented at the O-40 position of the hexosyl residue less two molecules of water. We believe this O-40 substitution is where the sulfate is in the original molecule. 2 amu, well within the precision of the API 2000 Mass Spectrometer (Fig. 88). Here we have a portion of the original ion that presumably had two substitutions hydrolyzed before the magnetic sector. We believe that the sulfate derivative and one sialic acid were hydrolyzed. 3 amu, we believe this structure to be correct (Fig.
It shows only one dihydrido sulfo group placed arbitrarily on O-40 of the galactose residue [22,25,26,39À42]. 70 This Figure is a MALDI spectrum of K casein O-linked oligosaccharide alditol/amino acid. Three out of the four ions are substituted by tyrosine, amino acid. Since tyrosine linked carbohydrates are not cleavable by β-elimination, they would persist. The remaining glycopeptide would be cleaved until the single amino acid remains. 72 This figure shows the structure found in the second MALDI-TOF of K casein O-linked oligosaccharide.
