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Instrumental Liquid Chromatography by N.A. Parris

By N.A. Parris

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0 does not adversely affect performance. If this proves to be general, the methods of separating fractions of support for packing columns will be greatly simplified. To overcome the variation of support being added to the column and changes in the packing method mentioned above many prefer to employ a mechanical procedure. Machine-packed columns offer two distinct advantages in that they minimise column-tocolumn variation and remove the tedium which is associated with methodically packing a column by hand, thus ensuring that the technique of addition or bouncing does not vary during the course of packing the column.

This results in a lower than expected column performance. The point where any particular packing method no longer produces acceptable columns depends considerably on the nature of the material being loaded into the column for use as the chromatographic support. The literature contains numerous accounts of methods for packing columns with various types of chromatographic supports. Some methods work best with spherical particles and others with irregularly shaped particles. Unfortunately many appear to give poor reproducibility, particularly from operator to operator.

It is conceivable that this approach may be investigated at some future date. These flow path inequalities are dependent largely on the uniformity of column packing and the diameter of the packing material used. To minimize this effect the mean particle diameter of the packing should be as small as possible consistent with obtaining a uniformly packed bed. This contribution to band broadening is essentially independent of mobile phase velocity and hence is a constant contribution to the overall plate height of a column.

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