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Essential Oils Analysis by Kubeczka

By Kubeczka

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Matrix effect can seriously degrade the accuracy of LC-MS analysis results. Method performance parameters such as limit of detection, limit of quantification, linearity, accuracy and precision, are affected by the loss of sensitivity and selectivity. Taking matrix effect into account (without actually reducing its influence) and thus improving the accuracy of the results can be done via standard addition [5], echo-peak technique [89], isotope dilution [42], postcolumn standard infusion [100], internal standard usage [52], and matrix matched calibration [106,107,108].

This strategy is based on the prior knowledge of the analyst concerning the biological system (commonly obtained by nontarget analysis) and, hence, is rather performed by targeted analysis and optional subsequent multivariate data analysis. In contrast, food fingerprinting techniques do not deal with the identification of all metabolites, but on the recognition of patterns, the so-called fingerprints of the matrix. MS has capacity to provide valuable information in diverse aspects related to food safety and quality, such as metabolites identification, contaminants determination, proteomics, lipidomics, glycomics, and foodomics.

79] with permission of Elsevier. 1 Liquid Chromatography–Mass Spectrometry (LC-MS) LC-MS is one of the most used combinations of chromatography and mass spectrometry. There are already several reviews that highlight the role LC-MS is playing within the field of food safety and quality [3,14]. LC-MS is one of the fastest developing fields in science and industry. Table 4 shows some of the most important application within food safety and quality. Analytical results are grouped together based on the type of chemicals analyzed (lipids, carbohydrates, glycoproteins, vitamins, flavonoids, mycotoxins, pesticides, allergens, and food additives) [4–6,9,30,39,42,44,46,47,50–53,80–123].

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