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For detection of real ASPV-infected samples, ASPV-positive and ASPV-negative plant extracts were tested. Fig. 10 shows the response curves for the samples at different dilutions. The SPR signal predictably decreases upon a decreasing concentration of virus, indicating that this is a feasible method for virus detection in real samples. 3. Conclusion Aptamers are proving to be effective molecular recognition probes for the analysis of high priority food contaminants. Aptamer-based biosensor platforms generally show sensitivities and specificities that could allow them to be competitive with existing detection methods.
With this sequence, Wang et al. developed a chromatographic strip assay method for rapid OTA detection (L. , 2011b). The aptamer-based strip assay was based on the competition for the aptamer modified with gold nanoparticles (GNPs) as the visual reporter between ochratoxin A and DNA probes. GNPs were prepared and thiolated aptamers (Aptamer: 5'-GAT CGG GTG TGG GTG GCG TAA AGG GAG CAT CGG ACA AAA AAA AAA AAA AAA AAA-SH-3') were self-assembled on the nanoparticle surface. DNA probes (Test line DNA probe 1: 5'-Biotin-CTA GCC CAC ACC CAC CGC ATT TCC CTC GTA GCC TGT-3' Control line DNA probe 2: 5'-Biotin-TTT TTT TTT TTT TTT TTT-3.
Ikanovic et al. selected a DNA aptamer for Bacillus thuingiensis (BT) spores with eight rounds of selection. The aptamer was then applied to produce a novel, solution-based sensor by using quantumdot reporter molecules for detection via fluoresence spectroscopy. BT spores are closely related to the food poisoning agent B. cereus so detection of BT demonstrates potential crossover applications to its foodborne relative. C. jejuni, a species of gram-negative bacteria commonly found in animal feces, is one of the most common causes of human gastroenteritis and has been linked with subsequent development of Guillain-Barré syndrome.