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Chemical Derivatization in Liquid Chromatography by J.F. Lawrence and R.W. Frei (Eds.)

By J.F. Lawrence and R.W. Frei (Eds.)

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11 B. DeVries, J. Amer. Oil Chem. ,40(1963) 184. 12 A. Berg and J. Lam, J. , 16(1964) 157. H. L. A. Fryer,J. , 89(1974)49. G. Harvey and M. Halonen, J. , 25(1966)294. P. Sharma and S. Ahuija, Z. Awl. , 367(1973)368. H. Shenk, Organic finctiowl Group Analysis, Pergamon Press, Oxford, 1968. 17 K. Groningsson and G. Schill, Acta. Phurm. Suecica, 6(1969)447. 18 S. Eksborg and C. Schill, Anal. G e m . , 45 (1973) 2092. A. Persson, Actu. Phurm. Suecicu, 5 (1968)343. A. Persson, Acta. Phurm. Suecica, 8(1971)193.

3. A “sandwich” chamber. modification of the multiple development technique frequently used in ascending TLC in order to improve separations. This consists of developing the plate to a certain distance, drying the plate and repeating the process one or more times. The advantage of the PMD system is its speed and separation efficiency. 4. The TLC plate is enclosed in a “sandwich” chamber which is placed in the solvent reservoir. Chromatography is permitted to proceed for 10-100 sec, after which time the TLC plate is heated by the heat radiator.

107 . . . . 107 . . . 107 . . . 107 . . . . 108 . . . . 108 . . . 1 General Many types of TLC equipment are commercially available and may serve a variety of purposes. Quantities of samples ranging from milligrams (for preparative work) to nanograms may be separated and detected depending on the sue of TLC plate. the type and 42 INSTRUMENTATION thickness of the layer and the choice of solvent system. Several practical aspects of the preparation for TLC analysis are discussed in the following sections.

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