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Cancer Cytogenetics: Methods and Protocols (Methods in by John Swansbury

By John Swansbury

A set of key cytogenetic and FISH recommendations utilized by smooth scientific laboratories within the genetic research of human malignancies. The book's functional recommendation and strategies are appropriate to be used at each point of craftsmanship, together with totally proven laboratories, yet with a sympathetic bias in the direction of someone contemplating constructing a brand new cytogenetics provider. the following the reader will locate not just straightforward tutorials at the basics of human karyotypes and chromosome research, but additionally certain discussions on how laboratories might optimally improve their repertoire of features to incorporate such more recent complementary options as CGH, FISH, and M-FISH.

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Extra resources for Cancer Cytogenetics: Methods and Protocols (Methods in Molecular Biology)

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Haematol. 76, 27–32. 8. Huntly, B. , Reid, A. , Bench, A. , et al. (2001) Deletions of the derivative chromosome 9 occur at the time of the Philadelphia translocation and provide a powerful and independent prognostic indicator in chronic myeloid leukemia. Blood 98, 1732–1738. 9. Heim, S. and Mitelman, F. (1995) Cancer Cytogenetics, 2nd edit. Pub. A. R. Liss, New York, p. 38. 10. , et al. (1999) Isochromosome 17q in blast crisis of chronic myeloid leukemia and in other hematologic malignancies is the result of clustered breakpoints in 40 11.

The quoted breakpoints on chromosomes 15q and 17q vary widely among different publications; the author favors those proposed by Stock et al. (22). The effect of the presence of secondary abnormalities is uncertain. In one study (23) (in which all secondary abnormalities were combined) they appeared to have no effect, but in others (25,26) the co-occurrence of trisomy 8 reduced the prognosis from good to standard. It would seem reasonable to expect that different secondary abnormalities have a different effect on prognosis.

8. Huntly, B. , Reid, A. , Bench, A. , et al. (2001) Deletions of the derivative chromosome 9 occur at the time of the Philadelphia translocation and provide a powerful and independent prognostic indicator in chronic myeloid leukemia. Blood 98, 1732–1738. 9. Heim, S. and Mitelman, F. (1995) Cancer Cytogenetics, 2nd edit. Pub. A. R. Liss, New York, p. 38. 10. , et al. (1999) Isochromosome 17q in blast crisis of chronic myeloid leukemia and in other hematologic malignancies is the result of clustered breakpoints in 40 11.

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